urea colorimetric assay hydrolyzed gelatin

#Urea colorimetric assay hydrolyzed gelatin Offline#

Ureolytic bacteria are used for microbial induced calcite precipitation (MICP). The assay could be adapted to other enzymes, which have a strong impact on the pH value.

The assay allows for straightforward, non-hazardous, parallelized, cheap and reliable measurements, making research on ureolytic bacteria for Microbial Induced Calcite Precipitation more efficient. The assay was developed so that the culture samples turbidity, salinity or buffer concentration does not have a negative impact on the fluorescence signal. Automated measurement is possible, as no sample preparation such as centrifugation or adjusting of the optical density is required.

#Urea colorimetric assay hydrolyzed gelatin Offline#

In both measurement systems, the fluorescence signal slope highly correlates with the urease activity measured offline with standard methods. The fluorescence signal can be measured with a microplate reader or with the microbioreactor system BioLector, allowing for automated urease activity measurements during cultivation experiments. After sample addition, the microbial urease hydrolyses urea, which results in a pH and hence fluorescence increase. The assay reagent consists of the green fluorescent pH-indicator fluorescein, urea and a phosphate buffer. The method is also suitable for automated measurements during microbioreactor experiments. A new urease activity microplate assay using a fluorescent pH indicator is presented. Quantifying urease activity is an important task for Microbial Induced Calcite Precipitation research. 2Chair of Biochemical Engineering (AVT.BioVT), RWTH Aachen University, Aachen, Germany.

1Munich University of Applied Sciences HM, Munich, Germany.Lapierre 1* Isabel Bolz 1 Jochen Büchs 2 Robert Huber 1*